abstract：:Salmonella typhimurium SL3261 (aroA mutant) expressing a recombinant Mycoplasma hyopneumoniae antigen was used to orally immunize swine against porcine enzootic pneumonia. This construct, designated S. typhimurium aro A SL3261 (pKF1), expressed a recombinant protein containing the carboxy-terminal 11 kDa of a 42 kDa M...

journal_title：Microbial pathogenesis

authors： Fagan PK,Walker MJ,Chin J,Eamens GJ,Djordjevic SP

更新日期：2001-02-01 00:00:00

abstract：:In this study we report the complete nucleotide sequence and genetic organization of the she pathogenicity island (PAI) of Shigella flexneri 2a strain YSH6000T. The 46 603 bp she PAI is situated adjacent to the 3' terminus of the pheV tRNA gene and includes an imperfect direct repeat of the 3'-terminal 22 bp of the ph...

journal_title：Microbial pathogenesis

authors： Al-Hasani K,Rajakumar K,Bulach D,Robins-Browne R,Adler B,Sakellaris H

更新日期：2001-01-01 00:00:00

abstract：:The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the s...

journal_title：Microbial pathogenesis

authors： Mohamed N,Visai L,Speziale P,Ross JM

更新日期：2000-12-01 00:00:00

abstract：:The 26 kDa protein of Helicobacter pylori, with 67% amino acid identity to alkyl hydroperoxide reductase (AhpC) of Campylobacter jejuni, was studied. We wanted to evaluate it the protein has AhpC activity. Therefore, an Escherichia coli mutant defective for alkyl hydroperoxide reductase and a plasmid expressing the 26...

journal_title：Microbial pathogenesis

authors： Lundström AM,Bölin I

更新日期：2000-11-01 00:00:00

abstract：:HrpA was found as a ribosome-associated protein which appeared in heat-stressed Mycobacterium bovis Bacillus Calmette-Guérin. Here, we have studied the function of HrpA in vitro. HrpA is a heat shock protein belonging to a small heat shock protein family. The putative molecular mass was 17784.86 kDa. Recombinant HrpA ...

journal_title：Microbial pathogenesis

authors： Tabira Y,Ohara N,Yamada T

更新日期：2000-10-01 00:00:00

abstract：:The corticosteroid-treated animal is well established as an experimental model for the study of Pneumocystis carinii pneumonitis (PCP). Latent or acquired infection with P. carinii in the murine lung progresses to fatal pneumonitis when the host is profoundly immunocompromized. In this study the effects of five immuno...

journal_title：Microbial pathogenesis

authors： Oz HS,Hughes WT,Rehg JE,Thomas EK

更新日期：2000-09-01 00:00:00

abstract：:The major limitation in histological examination of orally inoculated mice of Salmonella typhimurium has been the difficulty of attaining high enough levels for immunochemical detection. This problem has been solved by the use of confocal laser scanning microscopy (CLSM) analysis, which allows detection of bacteria in...

journal_title：Microbial pathogenesis

authors： Matsui H,Eguchi M,Kikuchi Y

更新日期：2000-07-01 00:00:00

abstract：:Mycobacterium tuberculosis (MTB) the causative organism of tuberculosis can remain dormant as a non-culturable organism, reactivate and cause disease in man and animals. There is a need for proof of viability of such organisms in order to understand the process of reactivation. PCR for bacterial DNA cannot distinguish...

journal_title：Microbial pathogenesis

authors： Pai SR,Actor JK,Sepulveda E,Hunter RL Jr,Jagannath C

更新日期：2000-06-01 00:00:00

abstract：:Repetitive tetranucleotide sequences of 5'-(CAAT)(n)-3' have been identified at the 5' end of an open reading frame (ORF) named lob1 from Haemophilus somnus strain 738. Based on sequence analysis, lob1 has 59% DNA homology to lex2B, which is involved in lipooligosaccharide (LOS) biosynthesis in H. influenzae. We now r...

journal_title：Microbial pathogenesis

authors： McQuiston JH,McQuiston JR,Cox AD,Wu Y,Boyle SM,Inzana TJ

更新日期：2000-05-01 00:00:00

abstract：:The goal of this study was to demonstrate the participation in cellular damage of a Trichomonas vaginalis proteinase with a molecular mass of 65 kDa (CP65). By two dimensional gelatin-gel electrophoresis of trichomonad proteins we detected four spots with proteolytic activity on the 65 kDa region, but only one, pI 7.2...

journal_title：Microbial pathogenesis

authors： Alvarez-Sánchez ME,Avila-González L,Becerril-García C,Fattel-Facenda LV,Ortega-López J,Arroyo R

更新日期：2000-04-01 00:00:00

abstract：:Monocyte chemoattractant protein-1 (MCP-1), a chemoattractant for monocytes, is presumed to play a pivotal role in the recruitment and accumulation of monocytes in various diseases including pulmonary infections. We examined here whether or not Pseudomonas nitrite reductase (PNR), a recently identified IL-8 inducer in...

journal_title：Microbial pathogenesis

authors： Sar B,Oishi K,Wada A,Hirayama T,Matsushima K,Nagatake T

更新日期：2000-01-01 00:00:00

abstract：:Vibrio cholerae elaborates zonula occludens toxin (Zot), a protein that increases the permeability of small intestinal mucosa by opening intercellular tight junctions. The zot gene is located, together with the genes encoding CT and Ace enterotoxins, within the genome of V. cholerae filamentous phage CTXsmall ef, Cyri...

journal_title：Microbial pathogenesis

authors： Uzzau S,Cappuccinelli P,Fasano A

更新日期：1999-12-01 00:00:00

abstract：:The transferrin-binding proteins TbpA and TbpB from Pasteurella haemolytica biotype A serotype 1 were tested for their ability to confer protection against experimental P. haemolytica infection when administered to calves in vaccine formulations containing one or both antigens. Vaccine groups included TbpB (single imm...

journal_title：Microbial pathogenesis

authors： Potter AA,Schryvers AB,Ogunnariwo JA,Hutchins WA,Lo RY,Watts T

更新日期：1999-10-01 00:00:00

abstract：:The prominent and phenotypically variable immunogenic protein of Trichomonas vaginalis, termed P270, is present in all isolates. Most, if not all, patients make antibody to the DREGRD epitope contained in the 333 bp tandemly repeating element (TRE). The complete sequence of p270 of a fresh clinical isolate was recentl...

journal_title：Microbial pathogenesis

authors： Musatovova O,Alderete JF

更新日期：1999-08-01 00:00:00

abstract：:Staphylococcus aureus is a bacterial pathogen causing approximately 80% of all cases of human osteomyelitis. This bacterium can adhere to and become internalized by osteoblasts and previous studies indicate that osteoblasts are active in the internalization process. In the current study, we examined the roles of micro...

journal_title：Microbial pathogenesis

authors： Ellington JK,Reilly SS,Ramp WK,Smeltzer MS,Kellam JF,Hudson MC

更新日期：1999-06-01 00:00:00

abstract：:Nonencapsulated Haemophilus influenzae frequently persists in the lungs of chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF) patients for prolonged periods of time. The bacteria are not eradicated by antibiotic treatment of the patients or by specific antibodies that are found in the sputum and ser...

journal_title：Microbial pathogenesis

authors： van Schilfgaarde M,Eijk P,Regelink A,van Ulsen P,Everts V,Dankert J,van Alphen L

更新日期：1999-05-01 00:00:00

abstract：:Two-component regulatory proteins, histidine kinases and response regulators, function in bacteria as sensing and adaptive factors in response to a wide range of environmental stimuli. Conserved histidine and glycine regions of histidine kinase sensor proteins were used to design degenerate oligonucleotide primers for...

journal_title：Microbial pathogenesis

authors： Haydel SE,Dunlap NE,Benjamin WH Jr

更新日期：1999-04-01 00:00:00

abstract：:We investigated the effects of vacuolating cytotoxin (VacA) prepared from Helicobacter pylori on the metabolism of gastric epithelial cells, AZ-521. VacA caused the ATP levels to decrease in a time-dependent manner; by approximately 20% in 6 h, 35% in 12 h and 50% in 24 h, at a concentration of 120 nM. This decrease w...

journal_title：Microbial pathogenesis

authors： Kimura M,Goto S,Wada A,Yahiro K,Niidome T,Hatakeyama T,Aoyagi H,Hirayama T,Kondo T

更新日期：1999-01-01 00:00:00

abstract：:Pasteurella haemolytica leukotoxin (LKT) is a member of the RTX family of pore-forming toxins that kill bovine immune cells. Several studies have suggested that RTX toxins kill target cells by the induction of apoptosis. In the present study, BL3 bovine leukaemia cells were exposed to LKT and assessed by molecular and...

journal_title：Microbial pathogenesis

authors： Wang JF,Kieba IR,Korostoff J,Guo TL,Yamaguchi N,Rozmiarek H,Billings PC,Shenker BJ,Lally ET

更新日期：1998-12-01 00:00:00

abstract：:Many pathogens of the Pasteurellaceae and Neisseriaceae possess a surface receptor that binds transferrin (Tf) as an initial step in an iron acquisition process. This receptor is comprised of two proteins, transferrin binding protein A (TbpA) and transferrin binding protein B (TbpB). Since the ability to recognize the...

journal_title：Microbial pathogenesis

authors： Retzer MD,Yu Rh,Zhang Y,Gonzalez GC,Schryvers AB

更新日期：1998-10-01 00:00:00

abstract：:Escherichia coli heat-stable enterotoxin Ip (STIp) is a typical extracellular toxin consisting of 18 amino acid residues synthesized as a precursor of pre (amino acid residues 1 to 19), pro (amino acid residues 20 to 54), and mature (amino acid residues 55 to 72) regions. STIp synthesized in the cytoplasm must cross t...

journal_title：Microbial pathogenesis

authors： Yamanaka H,Nomura T,Fujii Y,Okamoto K

更新日期：1998-09-01 00:00:00

abstract：:Ehrlichia risticii, the causative agent of Potomac horse fever, is an obligate intracellular rickettsial organism. To understand the role of 55 and 51 kilodalton immunodominant antigens of E. risticii in strain variation, their genes from the 25-D and 90-12 strains were cloned, sequenced, and expressed in E. coli. Seq...

journal_title：Microbial pathogenesis

authors： Vemulapalli R,Biswas B,Dutta SK

更新日期：1998-06-01 00:00:00

abstract：:Escherichia coli heat-stable enterotoxin b (STb) causes severe diarrhoea in weaning piglets. STb most probably has to bind to intestinal epithelial cells in order to achieve its effect. Using biotinylated biologically active STb, we developed a semi-quantitative binding assay using indirect fluorescence microscopy. We...

journal_title：Microbial pathogenesis

authors： Rousset E,Harel J,Dubreuil JD

更新日期：1998-05-01 00:00:00

abstract：:Activation of human gamma delta T cells by culture supernatants of virulent and avirulent mycobacteria was examined. The stimulatory potential of mycobacteria was influenced by the type of culture media and independent from their virulence. Activation of gamma delta T cells by phagocytes infected with viable virulent ...

journal_title：Microbial pathogenesis

authors： Schoel B,Kaufmann SH

更新日期：1998-03-01 00:00:00

abstract：:As an adaptation to the iron-restricted environment of the host, some bacterial pathogens possess iron acquisition pathways mediated by surface receptors that specifically bind transferrin from the host. The receptor is composed of two receptor proteins, TbpA and TbpB, which are both capable of binding to transferrin....

journal_title：Microbial pathogenesis

authors： Fuller CA,Yu R,Irwin SW,Schryvers AB

更新日期：1998-02-01 00:00:00

abstract：:Binding of human enterotoxigenic Escherichia coli (ETEC) to the small intestine is a prerequisite for colonization and is mediated by colonization factor (CF) antigens. Coli surface antigen 6 (CS6) is considered a CF but binding to isolated enterocytes has not been established. In this study bacteria expressing CS6 we...

journal_title：Microbial pathogenesis

authors： Helander A,Hansson GC,Svennerholm AM

更新日期：1997-12-01 00:00:00

abstract：:The gene (ystB) encoding the novel subtype of the heat-stable enterotoxin (Y-STb) was cloned from the chromosome of a clinical isolate of Yersinia enterocolitica 84-50 (serotype O:5, biotype 1A) and the nucleotide sequence was determined. The ystB contained 216 base pairs that encoded a protein of 71 amino acid residu...

journal_title：Microbial pathogenesis

authors： Ramamurthy T,Yoshino Ki,Huang X,Balakrish Nair G,Carniel E,Maruyama T,Fukushima H,Takeda T

更新日期：1997-10-01 00:00:00

abstract：:In the amino acid (AA) sequences of alpha antigen from mycobacteria, C-terminal regions were variable among a variety of mycobacterial species though the N-terminal regions were relatively conserved. These regions may possess some species-specific antigenic determinants of the alpha antigen from Mycobacterium scrofula...

journal_title：Microbial pathogenesis

authors： Takano M,Ohara N,Naito M,Matsumoto S,Matsuo T,Shirai R,Mizuno A,Yamada T

更新日期：1997-08-01 00:00:00

abstract：:We obtained mutants devoid of the O1-antigen, the capsular polysaccharide (K antigen) or both from Klebsiella pneumoniae clinical isolates (urinary infection). These mutants were grown in urine, and their ability to fimbriate and to adhere were studied. Mutants lacking the O1-antigen, independently of the other surfac...

journal_title：Microbial pathogenesis

authors： Merino S,Rubires X,Aguilar A,Tomás JM

更新日期：1997-07-01 00:00:00

abstract：:The focus of this study was the phenotypic characterization of Salmonella typhimurium mutants lacking the function of the response regulator mviA. The inactivation of mviA+ (mviA::kan) is shown to induce a significant change in the growth of most virulent strains, as reflected in the size of the colonies formed on aga...

journal_title：Microbial pathogenesis

authors： Swords WE,Giddings A,Benjamin WH Jr

更新日期：1997-06-01 00:00:00

abstract：:Inflammatory cytokines have been described to play a critical role in the orientation and amplification of the IgA immune response. In this study, we show that the intranasal administration of a Bordetella pertussis strain expressing the protective antigen glutathione-S-transferase of Schistosoma mansoni (Sm28GST) ind...

journal_title：Microbial pathogenesis

authors： Remoué F,Poulain-Godefroy O,Mielcarek N,Pierce R,Capron A,Locht C,Riveau G

更新日期：1997-05-01 00:00:00

abstract：:A DNA fragment encoding an approximately 18 kDa protein from Brucella abortus strain 2308 was cloned and expressed in Escherichia coli. This recombinant protein, designated BA18K, reacted in Western blot analysis with sera obtained from experimentally and naturally infected animals including mice, goats, dogs and huma...

journal_title：Microbial pathogenesis

authors： Kovach ME,Elzer PH,Robertson GT,Chirhart-Gilleland RL,Christensen MA,Peterson KM,Roop RM 2nd

更新日期：1997-04-01 00:00:00

abstract：:Mycobacterial antigens were detected in a cell surface ELISA on murine dissociated Schwann cells infected with Mycobacterium leprae. The time kinetics of expression and its refractoriness to modulation with monensin suggests that the antigens are likely to be integrated into the membrane during bacterial entry. This m...

journal_title：Microbial pathogenesis

authors： Birdi TJ,D'Souza S,Antia NH

更新日期：1997-03-01 00:00:00

abstract：:The exfoliative toxin produced by Staphylococcus hyicus strain 1289D-88 was purified as a single protein of approximately 30 kDa. Extracellular proteins of S. hyicus grown under small scale fermentation conditions were precipitated with ammonium sulfate. Separation of proteins was performed by hydrophobic interaction ...

journal_title：Microbial pathogenesis

authors： Andresen LO,Bille-Hansen V,Wegener HC

更新日期：1997-02-01 00:00:00

abstract：:In order to characterize the toxicity of the hemorrhagic toxin of Clostridium sporogenes isolated from the rabbit with antibiotic-associated hemorrhagic diarrhoea, we studied the toxin productivity of C. sporogenes cultured in various media and the toxic effect of the partially purified preparation of the culture supe...

journal_title：Microbial pathogenesis

authors： Hara-Kudo Y,Ogura A,Noguchi Y,Terao K,Kumagai S

更新日期：1997-01-01 00:00:00

abstract：:Elimination of pathogenic microorganisms in the liver may be an important effector mechanism in host defenses. In this paper we describe the adhesion, invasion and multiplication of Salmonella typhimurium in a murine embryonic hepatocyte cell line (ATCC TIB-73). Monolayers of hepatocytes treated with recombinant IFN g...

journal_title：Microbial pathogenesis

authors： Lajarin F,Rubio G,Galvez J,Garcia-Peñarrubia P

更新日期：1996-11-01 00:00:00

abstract：:Using whole bacteria, rather than extracted, purified DNA samples, we amplified the papE gene sequences from 63 Escherichia coli isolates belonging to O serogroups O1, O2 and O6. These isolates were from collections separated temporally as well as geographically: from four cities in the US and one in Sweden. PCR ampli...

journal_title：Microbial pathogenesis

authors： Jantausch BA,Hull SI

更新日期：1996-06-01 00:00:00

abstract：:Interleukin-8 (IL-8) is a neutrophil and T-lymphocyte chemotactic and activating factor. This cytokine is produced by many cell types including macrophages in response to a variety of microbial and non-microbial agents. In the present study, we determined the nucleotide sequence for bovine IL-8 cDNA. The amino acid se...

journal_title：Microbial pathogenesis

authors： Morsey MA,Popowych Y,Kowalski J,Gerlach G,Godson D,Campos M,Babiuk LA

更新日期：1996-04-01 00:00:00

abstract：:An inbred strain of the southern platyfish, Xiphophorus maculatus, was used as a host for Aeromonas hydrophila and Yersinia ruckeri infections. The infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent A. hydrophila or Y. ruckeri strains. Inoculating the platyfish in ...

journal_title：Microbial pathogenesis

authors： Kawula TH,Lelivelt MJ,Orndorff PE

更新日期：1996-02-01 00:00:00

abstract：:The 190 kDa outer membrane protein of Rickettsia rickettsii is a major immunodominant protective antigen which contains 13 tandem nearly identical repeating sequences. We have identified a deletion in the 190 kDa antigen gene of R. rickettsii strain Smith maintained in the laboratory. The deletion occurred within the ...

journal_title：Microbial pathogenesis

authors： Matsumoto M,Tange Y,Okada T,Inoue Y,Horiuchi T,Kobayashi Y,Fujita S

更新日期：1996-01-01 00:00:00